As an attractive model organism, C. elegans is widely used in many basic biological studies. Previously, insights into C. elegans biology were exclusively established with the analysis of a single reference strain (N2). Nowadays, research has begun to investigate genetic and phenotypic variation based on C. elegans wild isolates. Studies have demonstrated that the reference strain N2 exhibits laboratory adaptation extensively, while the natural populations of C. elegans show very little genetic differentiation worldwide. However, some of the marked phenotypic differences between C. elegans natural variation have been genetically and molecularly identified through quantitative genetic mapping approaches. The characterization of the nematode wild isolates elucidates C. elegans biology from a novel perspective. At CD BioSciences, we offer wild strain isolation and characterization services designed to facilitate and build knowledge for researchers using C. elegans as a research organism.
Although C. elegans is commonly referred to as a soil nematode, the worm has been widely isolated from various organic substrates above the soil layer, instead of soil samples. The decaying vegetal matter belongs to organic substrates including fruits, plant stems, and compost. Moreover, the nematode can also be isolated from a range of invertebrates, such as millipedes, isopods, and slugs. C. elegans isolates distribute worldwide, the characteristics of the distribution are correlated with temperature, and the species in temperate regions are more abundant than the tropical regions. Few C. elegans isolates are found in the tropics and mostly at high altitudes. Consistent with experimental data measured in the lab, the thermal optima of C. elegans is 25 °C and can't tolerate prolonged exposure above this temperature. Furthermore, it is notable that because of strong sampling bias, the current study of the global distribution of C. elegans is largely limited. There are beyond anthropogenic habitats, the more unperturbed sites such as forests for in-depth sampling and isolation of C. elegans isolates.
Fig.1 Geographic distribution of C. elegans wild isolates. (Poullet N, et al., 2015)
CD BioSciences has specialized in C. elegans research for many years, and our experienced scientists are confident in providing C. elegans wild strain isolation and characterization services to clients worldwide. C. elegans laboratory isolation is performed based on the samples you provide, followed by C. elegans characterization at the morphological and molecular level. Finally, the strain is cryopreserved using our proven C. elegans maintenance and storage techniques for rapid shipment to you. Our service process is as follows:
CD BioSciences is a professional C. elegans model service provider. Here, we offer isolate and identify C. elegans from their natural habitat to meet customers' needs for novel insights into C. elegans biology. We provide a one-stop solution of high-quality. If you have needs in this area, please contact us for more details.