The system is based on the detection of the movement of worms through the infrared light scattering effect. This system has a detection area composed of more than 20,000 infrared microbeams, each of 100 μm in width. This array of beams illuminates the microplate from the bottom, while a 6-camera video optical system (1M-pixel each) captures the light beams that pass through the sample. The software processes the analog signal, and the detection algorithm acquires the signal from each microbeam and processes it mathematically to detect the light scattering caused by the animals’ movement. When the system detects movement, the activity counter is incremented. The total calculated activity will be the integration of activity events in a time frame defined by the user.
The system has been validated for measuring multiple disease models and conducting microbiota experiments.
Obtaining results is very straightforward. The data is robust, reproducible, and free from user bias. It possesses immunity to bacterial interference up to OD600 = 1.0. Its expanded range of detection allows for the quantification of the movement of L1 worms (100 um in size) to larger parasites (>3 mm in size).