RNA Interference (RNAi) is widely used by researchers of the past decade in functional genomics. As one of the most important breakthroughs in molecular biology, RNAi technology allows us to directly observe specific gene silencing in eukaryotes. The phenomenon of RNAi is highly conserved among species. Since the groundbreaking discovery of RNAi in C. elegans, the model organism C. elegans has been widely used to study the mechanism of RNAi. With expertise and advanced technology, CD BioSciences revolutionizes the way you explore gene function and unravel biological mysteries, helping you to make breakthroughs in the field of molecular biology.
Extensive genetic and biochemical analysis revealed a two-step mechanism of RNAi-induced gene silencing. First, degradation of dsRNA into small interfering RNAs (siRNAs, 21~25 nt), by an RNase III-like activity. Second, the siRNAs join an RNase complex, RISC (RNA-induced silencing complex), which acts on the cognate mRNA and degrades it.
Fig.1 Schematic representation of RNAi mechanisms in C. elegans. (Grishok A., 2005)
Nowadays, RNAi has been applied to systematically shut down the expression of each gene to conduct genome-wide screens in C. elegans. For RNAi, double-stranded RNA (dsRNA) can be introduced in the worms by injection, soaking or feeding. RNAi screens by feeding are far less labor-intensive and inexpensive and are commonly used. dsRNA transcription can be ingested by C. elegans. This procedure is performed on petri plates or in liquid culture. RNAi by feeding in liquid culture is ideal for scoring easily observable phenotypes such as sterility, lethality, slow growth, and dramatic changes in GFP expression.
As a leading global provider of custom services, CD BioSciences is committed to providing you with high-quality C. elegans RNAi services, including targeted RNAi and RNAi screening services. With our innovative technology and expertise, we are confident that we can meet each of your specific needs with the following services.
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| Speak with our experts to determine an RNAi C. elegans model strategy. | Design and synthesize siRNAs/shRNA. | RNAi expression vector construction. | Introduce RNAi into C. elegans by feeding E. coli. | Screening and identification (via PCR, WB, or luciferase assay). | Deliver the final C. elegans model and validation report. |
CD BioSciences offers commercially synthesized siRNAs to silence virtually any gene in C. elegans, dramatically accelerating the pace of your research. siRNAs services are performed by a group of specially trained and experienced scientists in a streamlined workflow. If you are interested in our services, please contact us for more details.
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