C. elegans was introduced into the laboratory in the 1970s and has since gained popularity as a model for studying host-bacteria interactions. One of the strengths of C. elegans as a model organism is that its intestine can be colonized by the very bacteria on which it depends. Quantification of the bacterial load in the C. elegans intestine is an important and readily available metric when studying host-bacteria interactions. As an expert in C. elegans research, CD BioSciences is committed to providing passionate researchers around the world with services to accurately quantify bacterial colonization proficiency in C. elegans, hoping to bring convenience to customers in the related research.
In nature, the free-living C. elegans is found in soil, rotting plants and compost pits, feeding on bacteria and fungi. In the particularly complex microbe-rich environment, these microbes play different roles for C. elegans, including food, parasites, pathogens, and possibly competitors. Characterization of the natural microbiome of C. elegans revealed that the bacterial species inhabiting C. elegans are similar, despite having different origins. Besides, the natural microbiome has a beneficial effect on worms' growth and resistance to stress. In particular, as a key model organism within biology, its transparent gut, rapid growth rate and its bacterial diet make C. elegans a powerful system to study host-bacteria interactions. For the research of bacteria-worm interaction, the quantification of the intestinal bacterial load is an indispensable part as well as detecting the ability of bacteria to colonize the animal's gut.
Fig.1 Schematic illustrating the protocol "Quantification of Bacterial Loads in C. elegans". (Walker A C, et al., 2022)
At CD BioSciences, we are proud to offer a comprehensive range of innovative solutions for researchers and scientists working on C. elegans. In terms of C. elegans intestine bacteria quantification, our services include, but are not limited to:
Isolation of Intestinal Bacteria
Here we apply the method of homogenization and serial dilution to isolate bacteria from C. elegans. In addition to minimizing the general microbial contaminations, we also take measures against the presence of microorganisms from the surface of the nematodes to avoid interference in the analysis of microbes from within the worm body.
Establishment of Microbiome
Synchronized C. elegans larvae are cultured with experimental microbiome for a certain time before applying the washing step to remove bacteria outside of the nematode gut. It is notable that C. elegans embryos extracted from hermaphrodites can be a new generation within exposing anew to a microbe using hypochlorite treatment.
We quantify bacterial colonization by calculating colony forming units (CFU) in C. elegans intestines. Besides, as a simple method to measure colonization by bacteria tagged with fluorescent markers, the GFP-expressing bacteria are utilized to assess the degree of intestinal colonization under fluorescence microscopy.
CD BioSciences has been focusing on C. elegans research for many years and is committed to providing high-quality C. elegans customized services and innovative products to customers worldwide. If you are interested in our services or have any other questions, please feel free to contact us. We look forward to collaborating with you on your new gravitational projects.