The establishment of transgenesis in model organisms is necessary to explore the function, expression, and subcellular localization of gene products. Since the discovery that the Drosophila mariner element Mos1 could mobilize in the C. elegans germline, methods for efficient Mos1-mediated transgene insertion have been developed and widely adopted.
Fig.1 Schematic overview of MosSCI.( Frøkjaer-Jensen C, et al, 2008) Mos1-mediated single-copy insertion (MosSCI) is a method used to insert a single copy of transgene into a specific genomic location. The method works by breaking a chromosome at a particular location by excising a Mos1 transposon. The excision creates a double-strand DNA break, following a repair process with DNA sequences flanking the Mos1 insertion site if a vector containing DNA template with homology nearby. Besides, a positive selection marker (typically unc-119) and fluorescent markers can be applied to identify extra-chromosomal arrays.
We have developed a systematic platform for transgenesis in C. elegans using the MosSCI method, and the workflow is shown below.
Workflows of C. elegans MosSCI service- CD BioSciences
CD BioSciences offers a broad and integrated portfolio of laboratory and manufacturing services, especially the C. elegans model customization services. We have developed a systematic platform of transgenesis in C. elegans using the MosSCI method. The highly developed mosSCI system is a key technique for applications such as synthetic reporters and rapid testing of genetic rescue constructs, especially when germline expression is required. If you are interested in our services and have any questions, please feel free to contact us for more details.
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