The establishment of transgenesis in model organisms is necessary to explore the function, expression, and subcellular localization of gene products. Since the discovery that the Drosophila mariner element Mos1 could mobilize in the C. elegans germline, methods for efficient Mos1-mediated transgene insertion have been developed and widely adopted.

How MosSCI Works

Fig.1 Schematic overview of MosSCI. (Frøkjær-Jensen C, et al., 2008)

Mos1-mediated single-copy insertion (MosSCI) is a method used to insert a single copy of transgene into a specific genomic location. The method works by breaking a chromosome at a particular location by excising a Mos1 transposon. The excision creates a double-strand DNA break, following a repair process with DNA sequences flanking the Mos1 insertion site if a vector containing DNA template with homology nearby. Besides, a positive selection marker (typically unc-119) and fluorescent markers can be applied to identify extra-chromosomal arrays.

Our Services

CD BioSciences offers a broad and comprehensive portfolio of laboratory and production services for C. elegans research, and we have developed a systematic platform for transgenesis in C. elegans using the MosSCI method, dedicated to meeting your diverse needs.

Workflow of C. elegans MosSCI Service

Establishment of transgene insertion strategy	Selected Mos1 alleles	Plasmid construction	Plasmid microinjection	Screening and confirmation	Deliver the final C. elegans model and validation report

Advantages of MosSCI Method

• Transgenes can be expressed at levels close to endogenous gene expression.
• Transgene insertions are stable.
• Transgene expression is possible in the germline.

Features of Our Services

CD BioSciences offers a broad and integrated portfolio of laboratory and manufacturing services, especially the C. elegans model customization services. We have developed a systematic platform of transgenesis in C. elegans using the MosSCI method. The highly developed mosSCI system is a key technique for applications such as synthetic reporters and rapid testing of genetic rescue constructs, especially when germline expression is required. If you are interested in our services and have any questions, please feel free to contact us for more details.

References

1. Frøkjær-Jensen C, Wayne Davis M, Hopkins C E, et al. Single-copy insertion of transgenes in Caenorhabditis elegans[J]. Nature genetics, 2008, 40(11): 1375-1383.

Related Services

• C. elegans CRISPR/Cas9 Service
• C. elegans MosDel Service
• C. elegans Array and Integration Service