Lysosomes are ubiquitous, dynamic, membrane-bound organelles responsible for macromolecule degradation and catabolite recycling in eukaryotic cells. Lysosomes play a critical role in maintaining cellular health and homeostasis in the body by orchestrating fundamental processes such as autophagy, waste disposal, and nutrient recycling. CD BioSciences provides C. elegans lysosomal assays to customers worldwide using advanced imaging technology to visualize and analyze lysosomal structures.
By serving as a center of degradation, recycling and signaling, lysosomes play vital roles to maintain cell and tissue homeostasis.
Lysosomes not only receive material via the endocytic pathway, but also through the secretory pathway that directly from the cytoplasm. The material received from endocytic and secretory, such as nucleic acids, lipids, proteins, and oligosaccharides, are degraded within lysosomes. Breakdown products are delivered to the processes of cellular anabolism or catabolism.
Lysosomes also act as a signaling hub to assemble coordinated cellular responses and related factor information via key regulatory modules docked on the lysosomal surface.
In addition, lysosomal function is associated with diverse fundamental processes, including cell surface receptor turnover, antigen processing, plasma membrane repair, ion storage, autophagy, and aging.
Lysosome-related organelles (LROs) are specialized, membrane-bound cellular compartments found in organisms from worms to humans and share many common features of canonical lysosomes. For C. elegans, the LROs known as gut granules, are the site of microscopic autofluorescence and can be accumulated with ages. Due to the characteristics of autofluorescence and birefringence, the LROs of worms are easily recognized under polarized microscopy. Previous studies uncovered those mutations can disrupt LRO function as well as age-dependent accumulation of autofluorescence. Additionally, C. elegans LROs also act as a cellular reservoir for zinc, in order to prevent toxicity of high dietary zinc.
Fig.1 The ultrastructure of lysosomes changes dramatically in wild type with age but is maintained well in daf-2. (Sun Y, et al., 2020)
As a leading global provider of customized services, CD BioSciences is confident in providing comprehensive and innovative solutions for scientists focused on C. elegans research. In terms of C. elegans lysosomal assays, our services include but are not limited to:
CD BioSciences is a specialized provider of custom C. elegans services. There is a good system for deciphering the endocytosis and other related pathways by detecting lysosomal combining with live imaging techniques. Here we provide C. elegans lysosomal detection service to assist customers in relevant areas. If you are interested in our services, please contact us for more details.