Lysosomes are ubiquitous, dynamic, membrane-bound organelles responsible for macromolecule degradation and catabolite recycling in eukaryotic cells. Lysosomes play a critical role in maintaining cellular health and homeostasis in the body by orchestrating fundamental processes such as autophagy, waste disposal, and nutrient recycling. CD BioSciences provides C. elegans lysosomal assays to customers worldwide using advanced imaging technology to visualize and analyze lysosomal structures.

Functions of Lysosomes

By serving as a center of degradation, recycling and signaling, lysosomes play vital roles to maintain cell and tissue homeostasis.

Lysosomes also act as a signaling hub to assemble coordinated cellular responses and related factor information via key regulatory modules docked on the lysosomal surface.

In addition, lysosomal function is associated with diverse fundamental processes, including cell surface receptor turnover, antigen processing, plasma membrane repair, ion storage, autophagy, and aging.

Lysosomes in C. elegans

Lysosome-related organelles (LROs) are specialized, membrane-bound cellular compartments found in organisms from worms to humans and share many common features of canonical lysosomes. For C. elegans, the LROs known as gut granules, are the site of microscopic autofluorescence and can be accumulated with ages. Due to the characteristics of autofluorescence and birefringence, the LROs of worms are easily recognized under polarized microscopy. Previous studies uncovered those mutations can disrupt LRO function as well as age-dependent accumulation of autofluorescence. Additionally, C. elegans LROs also act as a cellular reservoir for zinc, in order to prevent toxicity of high dietary zinc.

Fig.1 The ultrastructure of lysosomes changes dramatically in wild type with age but is maintained well in daf-2. (Sun Y, et al., 2020)

Our Services

As a leading global provider of customized services, CD BioSciences is confident in providing comprehensive and innovative solutions for scientists focused on C. elegans research. In terms of C. elegans lysosomal assays, our services include but are not limited to:

• Lysosomal Localization and Acidity Detection
  Dyes such as LysoTracker, Acridine Orange, and Lysosensor dyes in the typical acidic environment of lysosomes. Stained lysosomes are imaged using fluorescence microscopy to observe their localization and dynamics while enabling lysosomal acidity detection.
• Lysosomal Morphology Observation
  We offer services to exam adult C. elegans ' lysosome morphology. Lysosomal tubule length, number, and volume are the main considerations. Confocal microscopy and high voltage electron microscopy (HVEM) help researchers capture detailed three-dimensional images of C. elegans lysosomes.
• Lysosomal Degradation Activity Detection
  Cathepsin L (CPL-1) is an inactive pro-enzyme and converted to the active mature form through proteolytic removal of the pro-domain in lysosomes. Lysosomal degradative activity is analyzed by examining the processing of endogenous CPL-1.

Why Choose CD BioSciences?

CD BioSciences is a specialized provider of custom C. elegans services. There is a good system for deciphering the endocytosis and other related pathways by detecting lysosomal combining with live imaging techniques. Here we provide C. elegans lysosomal detection service to assist customers in relevant areas. If you are interested in our services, please contact us for more details.

Reference

1. Sun Y, Li M, Zhao D, et al. Lysosome activity is modulated by multiple longevity pathways and is important for lifespan extension in C. elegans[J]. elife, 2020, 9: e55745.

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