Targeted changes in the genome offer the ability to address fundamentally important questions. Genome editing tools based on the CRISPR-Cas9 system are a breakthrough technology, which can precisely modify a genome in a relatively simple manner. As a research expert in C. elegans, CD BioSciences offers CRISPR/Cas9-based gene editing services.
The system is RNA-based bacterial defense mechanisms designed to recognize and eliminate foreign DNA from invading bacteriophage and plasmids. They consist of a Cas endonuclease that is directed to cleave a target sequence by a guide RNA (gRNA). Therefore, the system can be co-opted to cleave any target sequence of choice by modifying the sequence of the gRNA, which is encoded by a "CRISPR/array" in the bacterial genome.
Fig.1 Schematic representation of the CRISPR-Cas9 genome editing approach in C. elegans. (Kim HM, et al., 2016)
CRISPR/Cas9 system was first adapted for C. elegans in 2013 and has been successfully applied to C. elegans nowadays. We have established an efficient genome editing platform for delivering genetically engineered C. elegans optimized to meet your specifications.
|Customized CRISPR/Cas9 gene editing strategy||Design and synthesize gRNA||CRISPR vector construction||Plasmid microinjection||Screening and verification||Deliver the C. elegans model and report|
CD BioSciences offers a series of CRISPR/Cas9 C. elegans model customization services that are part of our integrated portfolio of custom C. elegans model development services. We are committed to offering convenient and efficient transportation services with shorter turnaround time and lower prices. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us for more details.
* For research use only.