C. elegans DNA 6mA Analysis Service

DNA methylation is a main epigenetic modification that modifies the function of the genes and thus affects gene expression through altering the chromosomal structure, DNA conformation and so on. Previous studies have demonstrated it is a popular research object, involving in many biological fields, such as embryonic development, cancer, and the influence of environmental factors on epigenetic inheritance. At CD BioSciences, we offer customized services for C. elegans research. N6-methyldeoxyadenine (6mA) is one of the most prevalent DNA modifications in eukaryotic genomes. Utilizing our C. elegans DNA m6A analysis services provide researchers with valuable insights into epigenetic DNA modifications.

Introduction to DNA 6mA in C. elegans

DNA methylation is an important type of epigenetic modification, the most common types being 5-methylcytosine (5mC), 6-methyadenine (6mA), and 4-methylcytosine (4mC), greatly expanding the information content of DNA. However, DNA methylation was considered to be absent in C. elegans because of the lack of detectable DNA methylation on the fifth position of cytosine (5mC), which is one of the major regulatory mechanisms of epigenetic inheritance in many species. Previously, 6mA represents a part of restriction-modification systems, helping differentiate host genome from foreign DNA, and was believed to exist only in bacteria, protists, and other lower eukaryotes.

Immunofluorescence of C. elegans wildtype extracted germlines show 6mA in every nucleus.Fig.1 Immunofluorescence of C. elegans wildtype extracted germlines show 6mA in every nucleus. (Greer E L, et al., 2015)

With the recent advent of more sensitive detection techniques, N6-methyldeoxyadenine (6mA) has also been detected in several eukaryotic organisms, including the cryptic nematode Hidradenitis elegans. Although the abundance of 6mA is quite low in the eukaryotic genome, these findings raised the exciting possibility that 6mA might serve as a conserved epigenetic mark across phyla. As a dynamic and reversible modification, 6mA plays an important role in gene expression regulation, development, and various biological processes.

Our Services

With a team of experienced scientists, CD BioSciences is committed to providing comprehensive DNA 6mA analysis services to our global clients, providing information about C. elegans 6mA from different perspectives. Our services include but are not limited to:

Preliminary Analysis Service of 6mA in C. elegans

Preliminary Analysis Service of 6mA in C. elegans

  • Dot blotting assay: preliminary quantitative analysis of 6mA in gDNA.
  • Immunofluorescence (IF): study the localization, relative expression, and activation status of target proteins in fixed cells or tissues.

C. elegans 6mA Quantification by UHPLC-MRM-MS

C. elegans 6mA Quantification by UHPLC-MRM-MS

  • DUPLC-MRM-MS: The required samples are simple to prepare, do not require prior purification, and are capable of detecting relatively low levels of 6mA, making it a highly sensitive and specific analytical method.

Conjoint Sequencing Analysis Service of6mA in C. elegans

Conjoint Sequencing Analysis Service of 6mA in C. elegans

  • DMeDIP-Seq: genome-wide determination of 6mA abundance and distribution.
  • DRNA-Seq: Quantification of gene expression and differential gene expression analysis of C. elegans 6mA.

Workflow of Our Services

Workflow of Our Services

Sample Preparation: Cultivate C. elegans and collect worms.

DNA and Chromatin Isolation: Worm genomic DNA and/or chromatin were extracted from the samples.

DNA Methylation Analysis: Perform bisulfite treatment to convert unmethylated cytosines to uracils while leaving methylated cytosines unchanged.

Histone Modification Analysis: ChIP and ChIP-seq.

Data Analysis: Sequencing data are processed and analyzed using bioinformatics tools.

Validation and Functional Studies: Use experimental techniques to validate epigenetic changes and gene editing or RNAi.

Advatages of Our Services

  • Expertise: Our team consists of specialized scientists with extensive experience in epigenetic research.
  • Advanced Technology: We have an advanced and proven technology platform for epigenetic analysis.
  • Customized Solutions: As a leading global provider of customized services, CD BioSciences offers high-quality customized solutions to meet your different needs.
  • Fast Turnaround: All deliverables will undergo rigorous quality testing and are guaranteed to be delivered on time.

CD BioSciences has specialized in C. elegans research for many years, and our experienced scientists are confident that we can provide you with high-quality customized solutions to meet your different research needs and advance your research progress. If you are interested in our services or have any questions, please feel free to contact us.


  1. Greer E L, Blanco M A, Gu L, et al. DNA methylation on N6-adenine in C. elegans[J]. Cell, 2015, 161(4): 868-878.
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