As a widely used experimental technique in developmental biology and genetics, food clearance experiments are commonly used to study feeding behavior and physiological processes in C. elegans. Food clearance analysis in C. elegans has contributed to the fields of basic biology and genetics, aging, drug discovery, and toxicology. Although C. elegans is a powerful model for studying feeding genetics, food-related behavior, and metabolism, direct measurement of its bacterial food intake is challenging. As experts in C. elegans physiological analysis, CD BioSciences is confident that we can provide you with high-quality, cost-effective C. elegans food clearance analysis services that will break down your research barriers and advance your research.
Analysis of C. elegans food clearance can help researchers understand the effects of genetic or environmental factors on feeding efficiency.
Measurement of bacterial clearance with C. elegans. (Gomez-Amaro RL, et al., 2015)
CD BioSciences provides a powerful tool for your C. elegans food clearance analysis with two assays, including:
Measuring food intake by quantifying changes in bacterial optical density over time.
Measuring food uptake by tracking the synthesis of new proteins through a metabolic pulse labeling strategy called pulse feeding.
One of these pulse feeding techniques allows for a clear determination of how food composition determines protein synthesis by measuring new protein synthesis and is an ideal method for C. elegans food clearance analysis that can be used to determine how food affects aging and health.
Analysis of worm movement includes tracking the trajectories of individual worms, measuring their speed, and assessing their turning behavior and path length.
Quantification of Clearance Area and Clearance Rate
Measure the area of the mats removed by worms over time, and then calculate the clearance rate.
Image Acquisition and Processing
Images or videos of the agar platform with worms and moss are captured periodically, the worms and moss are segmented and identified using relevant image analysis software.
As experts in C. elegans research, CD BioSciences has the advanced equipment and experienced scientists to confidently provide you with C. elegans food clearance analysis services, so please feel free to contact us if you have any needs.