C. elegans Gene Knockin by CRISPR/Cas9

The emergence of CRISPR/Cas9-based gene editing technology has revolutionized biological science research and is rapidly becoming an indispensable experimental tool for researchers of genetic model organisms such as C. elegans. CRISPR/Cas9 can insert DNA fragments into the C. elegans genome for efficient targeted genome editing. Our C. elegans gene knockin services include tagging service and point mutation service.

Applications of CRISPR/Cas9 Knockin

As a valuable tool for a variety of biomedical research applications, CRISPR/Cas9 offers a wide range of possibilities for customizing various models of C. elegans and thus advancing scientific research.

Gene Function Studies: CRISPR/Cas9 knockin technology is a powerful tool for studying genetic interactions and complexity, allowing researchers to introduce mutations, reporter genes or functional progenitors into specific genomic loci in C. elegans, which allows researchers to study gene expression and regulation with greater precision and accuracy.
Disease Modeling: Using CRISPR/Cas9 knockin technology, disease-associated mutations or specific genetic modifications can be introduced into C. elegans. the precise localization of target trajectories allows the creation of C. elegans models that accurately mimic human disease states.
Drug Screening: In drug screening, C. elegans models are a simple and attractive model system. C. elegans models constructed by CRISPR/Cas9 knockin technology can be used for high-throughput screening experiments for drug discovery and genomics research.

Fig.1 Diagram of CRISPR-Cas9 genome editing methodology in C. elegans. (Xu S. 2015)

Our Services

At CD BioSciences, our experienced scientists are focused on providing customized solutions for our customers worldwide. We utilize cutting-edge CRISPR/Cas9 knockin technology to achieve high efficiency and precise target loci, confidently ensuring reliable results. Our services include but are not limited to.

C. elegans Tagging Service

C. elegans Tagging Service

Fluorescent Tagging Service: CD BioSciences uses CRISPR/Cas9 knockin technology to add fluorescent protein tags at endogenous sites. Your choice of fluorescent tags includes YFP, BFP, eGFP, mCherry, mOrange, mScarlet, etc.

Immunotag Tagging Service: Adding immunotags to your protein of interest not only does not cause any infection to gene expression levels, but also provides a favorable opportunity to accurately quantify protein levels.

C. elegans Point Mutation Service

C. elegans Point Mutation Service

The use of CRISPR/Cas9 gene knockin technology can achieve precise and small editing of target sites. With our C. elegans point mutation service, researchers can accelerate research and gain greater insight into the function and behavior of associated genes, including investigating disease-causing mutations, exploring enzyme binding sites, and making any specific mutations of interest.

Workflow of C. elegans Gene Knockin

	Designing gRNA: Design gRNA for the specific genomic locus to be introduced to quide Cas9 nuclease to the target site.

	Constructing Plasmids: Construct CRISPR/Cas9 plasmids containing the target genes using molecular cloning techniques.

	Microinjection: Inject the successfully constructed plasmid into C. elegans.

	Selection and Screening: Selection and screening are performed by PCR, DNA sequencing or phenotypic assay according to the nature of the gene modification.

	Establishment of Stable Strains

Why Choose Us?

Highly efficient CRISPR technology platform
Experienced and expert scientists
Innovative products and high-quality, hassle-free, cost-effective services
One-stop solution
Fast turnaround time

CD BioSciences has a highly efficient gene editing technology platform and professional scientific staff, making us a focused provider of custom service for C. elegans models. We are committed to providing convenient and efficient shipping services with shorter turnaround times. If you are interested in our services or have any additional questions, please feel free to contact us.

Reference

Xu S. The application of CRISPR-Cas9 genome editing in Caenorhabditis elegans[J]. Journal of Genetics and Genomics, 2015, 42(8): 413-421.

For research use only.

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