C. elegans Lifespan Analysis Service

Because many in vivo experiments using C. elegans can be completed in a relatively short period of time, C. elegans has become an outstanding model organism for the study of molecular genetics, developmental biology, neuroscience, and cell biology. In addition, C. elegans has become a key tool for studying physiological processes, including aging, stress resistance and immunity, because C. elegans lifespan testing takes only a short time. With a clear vision and high-throughput data collection methods for C. elegans lifespan analysis, CD BioSciences is confident that we can provide you with a high-quality service and ensure the accuracy of your results.

C. elegans Lifespan Analysis for Aging Research

Aging is a fundamental biological process characterized by the progressive deterioration and death of cellular components and organelles, but its mechanisms are not fully understood. C. elegans has provided extraordinary insights into aging research and has successfully uncovered many genes and pathways associated with aging. The precise measurable lifespan of C. elegans has proven to be a very useful and effective tool for assessing the effects of various genetic, physiological, and environmental factors on organismal aging.

Genetic Manipulation: C. elegans has a relatively simple and well-characterized genome that makes it an ideal organism for studying genetic factors that influence aging. Researchers have identified a number of longevity genes in C. elegans that regulate aging, and manipulation of these genes has led to a better understanding of the molecular mechanisms of aging.
Environmental Factors: Exposing C. elegans to different temperatures, dietary restrictions, oxidative stress, or other conditions enables the study of the effects of various environmental factors on aging by analyzing C. elegans lifespan. This helps to elucidate how external factors influence aging and provides valuable insights into strategies to promote longevity.

C. elegans lifespan assays are either performed with solid or liquid media. Fig.1 C. elegans lifespan assays are either performed with solid or liquid media. (Park HH, et al., 2017)

Our Services

In nematodes, lifespan is typically defined as the number of days an animal remains responsive to external stimuli, and animal death is considered when they fail to respond to external stimuli. CD BioSciences offers C. elegans lifespan analysis services on liquid or solid media, using advanced microfabricated equipment to automatically collect and analyze experimental data.

Workflow of Our Service

	Prepare NGM Plates: Prepare NGM plates supplemented with FUdR with or without the addition of a specific compound concentration. Plates are seeded with 10 times concentrated OP50 after plates are solidified.

	Prepare Synchronized Worms: Prepare the synchronized eggs and culture at 20 °C. Transfer L1 worms to the NGM plates with or without compound of specific concentration.

	Score Animals for Lifespan: Worms are transferred to plates with FUdR with or without compound of specific concentration when they reached L4 stages. Record the date and the number of worms that are dead every other day until all worms die.

	Delivery of Results: On-time delivery of final nematode longevity analysis results and validation reports.

Our Advantages


Advanced and high-throughput technology platform	Experienced and talented team of experts	Accurate and reliable experimental data	Change procedures to suit your requirements	High quality and cost-effective customization services

C. elegans lifespan analysis is a valuable tool in aging research, helping researchers gain insight into the effects of genetic and environmental factors on the aging process. CD BioSciences has a robust experimental platform for C. elegans lifespan analysis and is confident that we can provide you with high-quality, cost-effective C. elegans lifespan analysis services. All deliverable results will be rigorously validated and delivered on time. To improve the speed of your study results and success, please do not hesitate to contact us for more details.

Reference

Park H E H, Jung Y, Lee S J V. Survival assays using Caenorhabditis elegans[J]. Molecules and cells, 2017, 40(2): 90.

For research use only.

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