C. elegans Mating Behavior Analysis Service

Analysis of C. elegans mating behavior not only reveals the reproductive strategies of specific organisms, but also provides broader implications for understanding the sexual behavior and mechanisms of different species, with important implications for fields such as evolutionary biology, neuroscience, and reproductive biology. As experts in C. elegans research, CD BioSciences has a team of experienced scientists and researchers dedicated to providing you with customized solutions to help you advance your research. We leverage our expertise in C. elegans phenotyping to provide you with cost-effective analysis of C. elegans mating behavior.

Characterizing C. elegans mating behavior.Fig.1 Characterizing C. elegans mating
behavior. (Yu C C J, et al., 2013)

Introduction into C. elegans Mating Behavior

The key to mating behavior in C. elegans is mate recognition, where hermaphrodites release pheromones to attract males, and this chemical communication is the key signal that initiates the mating process. When a male detects a pheromone cue, he exhibits specific behaviors to locate and mate with the hermaphrodite:

  • Search Behavior: Males use sensory cues, especially the characteristic pheromones, to locate and track potential mates.
  • Contact and Recognition: Males find their targets and contact them by using their head or tail, which is an important step in initiating the mating process.
  • Tail Curvature: After contacting the hermaphrodite, males bend their tails ventrally, curling them ventrally, which allows the male to align his tail with the vulva of the hermaphrodite.
  • Spicule Insertion: After the male uses a mating structure called spicules to puncture the vulva, the needle-like body is inserted into the genital tract.
  • Sperm Transfer: Once the pinna is inserted, the male transfers sperm into the hermaphrodite's reproductive tract.
  • Post-mating Behavior: Males may detach from the hermaphrodite and move on to find more mates; hermaphrodites may exhibit behaviors such as vulva closure to prevent further sperm entry.

Each step of C. elegans mating behavior involves sensory perception, neural processing, and motor coordination.

Our Services

At CD BioSciences, we focus on providing cutting-edge services in C. elegans phenotype analysis, using a combination of genetic manipulation, imaging techniques, and behavioral analysis to dissect the complexity of C. elegans mating behavior. In the area of C. elegans mating behavior analysis, we offer the following service offerings:

Observation and Scoring: Mating behaviors of C. elegans, including male approach, tail curl, bone pin insertion and sperm transfer, were observed and recorded through microscopy.

Video Recording: High-resolution video microscopy was used to record the duration of each step in C. elegans mating behavior, the sequence of events, and the behavior exhibited by males and hermaphrodites.

Quantitative Analysis: Parameters measured include mating frequency, duration of mating, waiting time to start mating or number of successful mating.

Genetic Manipulation: production of mutants with specific genetic alterations or modifications to identify the genes and pathways involved in mating behavior.

Why Choose Us?

Expertise and Experience

Expertise and Experience





Fast Turnaround

Fast Turnaround

As a leading global provider of custom services, CD BioSciences not only offers you high quality services, but also has innovative products to accelerate your research progress. Please contact us to discuss how our C. elegans phenotyping services can contribute to your research goals.


  1. Yu C C J, Raizen D M, Fang-Yen C. Multi-well imaging of development and behavior in Caenorhabditis elegans[J]. Journal of neuroscience methods, 2014, 223: 35-39.
For research use only.

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