As a component of membranes, lipids act as auxiliary messengers and signaling sensors and are vital to life as they play an important role in energy storage. C. elegans lipid staining contributes to researchers' understanding of lipid biology, metabolism, and related diseases. By accurately assessing the level of lipid accumulation in C. elegans can help to understand the molecular mechanisms that regulate lipid metabolism. Several methods are already available to visualize and measure the amount of lipids in C. elegans. At CD BioSciences, our experienced scientists can offer you a wide range of C. elegans lipid staining services to meet each of your specific needs.
Lipid metabolism is a highly conserved process in eukaryotes. As researchers have become increasingly interested in the interactions between lipid metabolism and energy homeostasis, C. elegans has become a popular model system for studying lipid metabolism.
Many fat-soluble dyes, such as BODIPY, Nile Red, and Oil Red O (ORO) can be used to determine fat storage in C. elegans. Among them, ORO is an immobilizer-based dye that has been validated as an accurate method for the assessment of major fat stores in C. elegans. In contrast, Nile Red and BODIPY can be used to detect fat storage by feeding to live worms. Among them, Nile Red and ORO are inexpensive and reliable methods for quantitative measurement of lipid levels and qualitative observation of lipid distribution among tissues, respectively. These stains allow high-throughput screening of various lipid metabolism genes and pathways, and in addition, they reduce interactions with surrounding tissues due to their hydrophobic nature.
Fig.1 Labeled imaging of fat stores using Oil Red O and Nile Red, and Nile Red staining of fixed worms and Nile Red and BODIPY-labeled fatty acids fed to live worms. (Yen K, et al., 2010)
The abundance, size, and distribution of lipids in C. elegans can be easily assessed by staining methods. As a leading global provider of custom services, CD BioSciences offers you C. elegans lipid staining services including but not limited to:
Oil Red O
ORO is a lipid-soluble diazo oxide dye that detects neutral lipids and cholesterol esters. C. elegans stained with ORO shows red lipid droplets on its transparent body, which helps to qualitatively assess the distribution of lipids in different tissues.
Nile Red is a benzophenoxazone dye that is readily soluble in various organic solvents, but mostly insoluble in water. It is an excellent hemolysis pigment dye for staining neutral lipids and dissolves well in lipids.
BODIPY is a bright green, fluorescent dye with the chemical formula C9H7BN2F2 and has unique hydrophobic properties suitable for staining lipids, membranes, and other lipophilic compounds.
As a leading global provider of custom services, CD BioSciences has highly trained and experienced scientists who not only provide comprehensive, most effective C. elegans research solutions to our global clients, but also innovative products. Each of our deliverables is subjected to rigorous quality checks to ensure the reliability and accuracy of results. If you are interested in our services or have any additional questions, please feel free to contact us.