As an excellent model organism for genetic studies, C. elegans is widely used in the study of biological processes such as cell research, behavior, development, and aging. Targeted RNA interference (RNAi), a technique that specifically inhibits gene expression in a specific organism or tissue, has become a valuable tool for analyzing C. elegans gene function by introducing double-stranded RNA (dsRNA) into the organism, resulting in knockdown of the target gene. As a leading custom service provider specializing in C. elegans research, CD BioSciences, with our refined equipment and expertise, offers a comprehensive solution for C. elegans targeted RNAi to help our customers unravel more of their genetic mysteries.

Fig.1 The improved RNAi vector is more efficient than the standard vector. (Sturm Á, et al., 2018)

Introduction to C. elegans Targeted RNAi

RNAi has become an important tool for studying C. elegans because of its simplicity, efficiency, rapidity, and specificity for determining loss-of-function phenotypes of genes in C. elegans and has been the basis of several genome-wide screens. In addition, RNAi is also an excellent way to examine genetic interactions. For C. elegans targeted RNAi, the principle is to inactivate genes by targeting dsRNA to specific enzymes of endogenous mRNAs under the control of the C. elegans promoter.
The details of C. elegans Targeted RNAi are as follows:

• Design and Synthesis of dsRNA: Design and synthesis of dsRNA for mRNA targeting the target gene.
• RNAi in C. elegans: The L4440 plasmid containing the target gene is transformed into HT115 E. coli to feed C. elegans.
• Phenotypic Analysis: Observe C. elegans for phenotypic changes including, developmental defects, behavioral changes and altered physiological characteristics.

C. elegans targeted RNAi is an invaluable tool for studying gene function, identifying molecular pathways, and elucidating biological processes such as development, behavior, and disease, and has greatly contributed to researchers' understanding of gene regulation.

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CD BioSciences offers precise and efficient C. elegans targeted RNAi services that can help you unlock the mysteries of gene function.

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Reference

1. Sturm Á, Saskői É, Tibor K, et al. Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research[J]. Nucleic Acids Research, 2018, 46(17): e105-e105.

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• C. elegans RNAi Screening Service