C. elegans Cold Tolerance Analysis Service

Ambient temperature directly affects the biochemical processes of organisms and is critical to the survival and proliferation of animals. Animals therefore employ a range of mechanisms to cope with environmental changes. Cold tolerance in C. elegans is a novel concept compared to heat tolerance and is responsible for different neurons and signaling pathways. At CD BioSciences, we provide C. elegans cold tolerance analysis services to help customers understand the mechanism of cold adaptation and advance experimental progress.

Introduction into C. elegans Cold Tolerance

The growth rate and reproductive efficiency of many animals are affected by environmental changes. Most animals have evolved to cope with warming and cooling, developing mechanisms to adapt and tolerate temperature changes. Organisms adapt to changes in environmental temperature through a variety of mechanisms, or through endogenous homeostatic mechanisms, such as changes in fatty acid and phospholipid saturation of total lipids. C. elegans maintains cell membrane fluidity at low temperatures by increasing the overall proportion of saturated fatty acids. Temperature-sensing neurons (ASJ) of C. elegans detect temperature and control cold tolerance through insulin signaling.

  • During cold tolerance, ASJ detect temperature changes and release insulin that binds to the insulin receptor DAF-2 in the gut and neurons.
  • Insulin signaling regulates downstream gene expression mediated by DAF-16, a FOXO-type transcription factor.
  • Delta 9-desaturase then initiates a cold-induced lipid regulation response downstream of DAF-16.

A feedback network in cold tolerance of C. elegans.Fig.1 A feedback network in cold tolerance of C. elegans. (Sonoda S, et al., 2016)

C. elegans is suitable for cold tolerance research because of its simple nervous system, easy laboratory culture, and fast reproduction speed. In addition, C. elegans also exhibited morphological and behavioral changes in response to cold stress. Using C. elegans as a model, the behavior and mechanism of animal adaptation to environmental changes can be studied.

Our Services

C. elegans is an attractive model to study cold tolerance, not only capable of responding to changes in environmental temperature by changing morphology and behavior, but its molecular genetics are well established. CD BioSciences focuses on providing cutting-edge services in the field of C. elegans research. With our professional knowledge and rich experience, we are confident to provide you with customized solutions for C. elegans cold tolerance analysis to advance your research progress. The following are the key elements of my C. elegans cold tolerance analysis service:

Behavioral Analysis: Evaluate parameters such as movement and feeding behavior of C. elegans in cold conditions.

Survival Assays: Identify genetic or environmental factors affecting hardiness by measuring survival rates.

Fertility Analysis: Changes in temperature can affect C. elegans development and reproductive capacity, so assess cold conditions to examine various developmental stages.

Physiological Measurements: Evaluate hypothermia-induced physiological changes in C. elegans by measuring parameters such as metabolic rate, ATP levels, oxidative stress, or membrane fluidity.

Genetic and Molecular Analysis: Generate target strains according to your research needs to reveal specific genes and pathways involved in the cold stress response.

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As a C. elegans research specialist, CD BioSciences is committed to providing you with high-quality, cost-effective, customized services to advance your research. Collaborate with us and use our expertise to unlock more of the secrets of biological processes. If you are interested in our services or have any steady state, please feel free to contact us.


  1. Sonoda S, Ohta A, Maruo A, et al. Sperm Affects Head Sensory Neuron in Temperature Tolerance of Caenorhabditis elegans. Cell Rep. 2016; 16 (1): 56–65[R].
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