Proteomic analysis refers to the large-scale analysis of proteins in a particular biological sample under specific conditions. Methods used for proteomic analysis include mass spectrometry, 2D gel electrophoresis, and protein microarrays, which help researchers identify and quantify proteins, study their post-translational modifications, and understand their functions in organisms. CD BioSciences provides C. elegans proteomic analysis services to clients worldwide, helping you to gain a comprehensive understanding of the C. elegans proteome and unravel the complex mechanisms and interactions that underlie its biology.
As one of the excellent animal models, C. elegans is widely used in drug development and provides important insights for studies such as neurodegenerative diseases and metabolic syndrome. Because of its suitability for high-throughput screening, RNA interference, and the availability of thousands of mutants, most of the research on C. elegans has been conducted at the genetic level. However, in the post-genomic era, determining the cellular function of each gene product remains an unfinished goal. Mass spectrometry-based proteomics focusing on the actual gene products (i.e., proteins) can help researchers solve the puzzle.
Fig.1 SILAC-Based Deep Proteome Analysis Reproducibly Identifies 9,398 Proteins in C. elegans. (Narayan V, Ly T, et al., 2016)
C. elegans research utilizing forward or reverse genetic screening has focused primarily on genome-based approaches, and the proteins responsible for biological actions have been largely ignored. As instrumentation and technology continue to evolve, greater advances have been made in high-throughput proteomics analysis and data collection. For example, the introduction of dual-pressure linear ion trap mass spectrometers with higher sensitivity and faster cycle times has facilitated the detection and identification of proteins and unique peptides in the C. elegans proteome. For quantitative proteomics studies in cell cultures, Mann's group introduced "stable isotope labeling with amino acids in cell culture (SILAC)" in 2002.
At CD BioSciences, we are confident that we can provide our global clients with C. elegans proteomic analysis services that offer a unique window into the complexity of important biological processes such as development, aging, and disease. Our expert team of experienced scientists ensures that you get high-quality proteomic data to break through research challenges and advance your research. The methods we use for C. elegans proteome analysis include, but are not limited to:
Sample Preparation: The worms are homogenized to extract total proteins and the concentrations are measured. Proteins are separated using SDS-PAGE or 2D-PAGE.
Protein Digestion: Total protein is digested into peptides using proteases.
Mass Spectrometry (MS) Assay
Protein identification: Protein identification is performed using the C. elegans protein database.
Validation and Research: Validation of identified proteins using techniques such as WB, IP, and IF.
Whether you are exploring developmental mysteries, unraveling disease mechanisms or drug screening, CD BioSciences' C. elegans proteomic analysis services can advance your research. With our experienced scientists and advanced and mature technology platform, we are confident that we can provide you with high-quality customized solutions based on your specific needs. We guarantee that all deliverables will undergo rigorous quality testing and be delivered on time. If you are interested in our services or have any questions, please feel free to contact us. We look forward to working with you on your attractive project.