UV stress can have deleterious effects on organisms, including DNA damage, oxidative stress, and cellular dysfunction. Short wavelengths of light, especially ultraviolet light, cause avoidance behavior in C. elegans. This is mediated by a group of sensory neurons and is a protective mechanism to avoid lethal doses of UV light from sunlight. C. elegans, with its well-defined genetics and conserved cellular pathways, is an excellent model for studying the UV stress response. By utilizing our expertise and advanced technology, we have helped researchers gain insight into the molecular and cellular mechanisms of C. elegans' response to UV stress, which in turn provides valuable insights into organisms' responses to UV.
Light perception is critical to organisms, which have evolved various types of photoreceptors to detect light. For C. elegans, exposure to UV light leads to an escape behavior (avoidance behavior) and a pharyngeal pumping inhibition (acute cessation of feeding). In contrast to animals with external pigmentation, the worms are particularly vulnerable to the mutagenic effects of UV due to the transparent body that allows the penetration of lights. The LITE-1 taste receptor found in C. elegans, which is homolog as a UV-specific photoreceptor, and totally distinct from other photoreceptors in metazoans, microbes, and plants. The cellular response to UV irradiation in C. elegans is homologous to the mammalian DNA damage repair pathways, including:
Fig.1 The C. elegans taste receptor homolog LITE-1 is a photoreceptor. (Gong J, et al., 2016)
As a model organism commonly used in sensory perception, C. elegans detects and responds to a variety of sensory cues, such as mechanical forces (e.g., touch and stretch), chemicals (e.g., odorants and taste enhancers), and temperature. CD BioSciences specializes in C. elegans research, and our C. elegans UV stress analysis service is tailored to meet your specific research needs. The key features of our service are listed below:
Controlled UV Exposure: CD BioSciences delivers controlled and reproducible UV stress to C. elegans using precise UV irradiators to simulate different stress levels and study the organism's response.
DNA Damage Assessment: Specialized techniques such as star assay and immunofluorescence staining are used to measure and analyze the level of DNA damage in C. elegans.
ROS Measurement: Fluorescent probes such as dihydroethidium (DHE) or dichlorodihydrofluorescein diacetate (DCFDA) are used to measure ROS levels in C. elegans under UV stress.
Phenotype Analysis: As experts in C. elegans research, CD BioSciences has a wide range of C. elegans phenotyping programs to help you gain insight into the molecular and cellular mechanisms underlying the C. elegans response to UV stress.
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