C. elegans is a tiny nematode that is often used as a model organism for biological research due to its anatomical simplicity, short lifespan, and ease of manipulation. Histological analysis of C. elegans focuses on analyzing its tissues at the microscopic level in order to study development, morphology, and cellular structure, which is essential for studies in genetics, developmental biology, neurobiology, and disease mechanisms. At CD BioSciences, our experienced scientists utilize proven and advanced technology platforms to provide C. elegans histology services to clients worldwide, analyzing the histomorphology and metabolic changes of wild type and various mutant strains.

Introduction to C. elegans Histology

One of the attractive characters of C. elegans, a powerful model system, is that despite its simplicity, it has defined tissues. The nematode is often described as a concentric tube and the epidermis encloses a pseudocoelomic fluid-filled cavity housing the main organ systems, including digestive, reproductive, and excretory systems. Moreover, inside the epidermis are the bands of muscle, which control the movement of the organism. Previous studies have indicated that sectional histology can be applied to define novel phenotypes in C. elegans. Staining methods such as enzyme histochemistry combined with fresh frozen serial sections are used to analyze C. elegans metabolism. C. elegans histology provides a comprehensive picture of the metabolic state of a single worm and make a positive influence in understanding metabolic changes in C. elegans.

Fig.1 Pathobiology of aging C. elegans tissues. (McGee M D, et al., 2011)

Our Services

With a wealth of expertise and advanced technology, CD BioSciences is confident in providing comprehensive histological analysis services for C. elegans to clients worldwide. Our services include but are not limited to:

• H&E and PAS Staining
  Hematoxylin and eosin (H&E) staining is used as a common and widely used staining technique to show the structure of cells and tissues in a sample. Because different tissues exhibit specific patterns of hematoxylin and eosin binding, pathomorphological features are readily visible. In addition, periodic acid Schiff's (PAS) reagent is exploited to stain the carbohydrates in C. elegans tissues.
• Lipid Staining
  Many fat-soluble dyes, such as BODIPY, Nile Red, and Oil Red O (ORO) can be used to determine fat storage in C. elegans. ORO is applicable to whole-mount C. elegans samples for staining neutral fat deposits. The results exhibit as color image segmentation of ORO stained and hematoxylin counter-stained are reproducible and can be quantified to reflect overall lipid content in individual animals.

Why Choose CD BioSciences?

CD BioSciences partners with academic and corporate researchers around the world to provide high-quality C. elegans customization. All of our deliverables are subjected to rigorous quality testing to ensure accuracy. If you are interested in our services or have any other questions, please feel free to contact us.

Reference

1. McGee M D, Weber D, Day N, et al. Loss of intestinal nuclei and intestinal integrity in aging C. elegans[J]. Aging cell, 2011, 10(4): 699-710.

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