Host Species | Mouse |
Species Reactivity | C. elegans, Human, Mouse, Rat, Pig, Cattle, Chicken, Fruit fly, Equine, Plant, Primate, Zebrafish |
Conjugate | Unconjugated |
Clone Number | NW0114 |
Specificity | 8.5 kDa. |
Immunogen | Purified bovine erythrocyte ubiquitin and KLH cross-linked with glutaraldehyde. |
Format | Liquid or Lyophilized |
Purification | Affinity purified |
Product Overview | Mouse Anti-Zebrafish RPS27A (clone NW0114) Antibody is a Mouse antibody against the epitope of the Ubiquitin. It can be used for Ubiquitin detection in Western Blot,Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin. |
Availability | Made-to-order |
Target Name | RPS27A |
Gene ID | 6233 |
UniProt ID | P62979 |
Introduction | Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified. |
Application | IB, IF, IHC, IHC-Fr, IHC-P, WB |
Application Notes | WB: 1/1000-1/2000 IF: 1/2000 IHC: 1/2000 IHC-Fr: 1/2000 IHC-P: 1/2000 |
Shipping | Shipped on dry ice. |
Storage | Store at 4 °C: short-term (1-2 weeks) Store at -20 °C: long-term and future use |
Buffer | PBS, 50% Glycerol |
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