Host Species | Mouse |
Species Reactivity | C. elegans, Human, Mouse, Rat, Pig, Fruit fly, Yeast |
Conjugate | Unconjugated |
Clone Number | NW0085 |
Specificity | 15 kDa. |
Immunogen | Synthetic peptides in the N-terminal region of immunohistone H3. |
Epitope | AA 1-50 |
Format | Liquid or Lyophilized |
Cellular Localization | Nucleus |
Purification | Protein G purified |
Product Overview | Mouse Anti-Yeast HIST2H3C (AA 1-50, clone NW0085) Antibody is a Mouse antibody against the epitope (AA 1-50) of theHistone H3 [Trimethyl Lys9]. It can be used for Histone H3 [Trimethyl Lys9] detection in Western Blot, Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation. |
Availability | Made-to-order |
Target Name | HIST2H3C |
Gene ID | 126961 |
UniProt ID | Q71DI3 |
Introduction | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. |
Application | ChIP, ELISA, IF, IHC, IHC-P, IP, WB |
Application Notes | WB: 1/2000 ChIP: 1/10-1/500 ELISA: 1/100-1/2000 IF: 1/200 IHC: 1/1000 IHC-P: 1/1000 |
Shipping | Shipped on dry ice. |
Storage | Store at 4 °C: short-term (1-2 weeks) Store at -20 °C: long-term and future use |
Buffer | Tris-glycine, 0.15M NaCl |
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