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| Host Species | Mouse |
| Species Reactivity | C. elegans, Human, Mouse, Rat, Budding Yeast, |
| Conjugate | None |
| Clone Number | MMB5 |
| Immunogen | The RNA pol II antibody was raised against the synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes the phosphorylated and non-phosphorylated forms of the largest subunit of RNA pol II. |
| Format | Liquid |
| Purification | Affinity purified |
| Availability | Made-to-order |
| Target Name | RNA pol II |
| Gene ID | 32100 |
| Introduction | RNA pol II (RNA polymerase II) is responsible for the synthesis of messenger RNA in eukaryotes. RNA pol II contains a carboxy-terminal domain consisting of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues, which are phosphorylated in the actively transcribing RNA polymerase. In addition, RNA pol II combines with several other polymerase subunits to form the DNA binding domain of the polymerase, in which the DNA template is transcribed into RNA grooves. |
| Application | ChIP, IF, WB |
| Application Notes | ChIP: 10 µL per ChIP ChIP-Seq: 20 µL each WB: 1:2000-1:5000 The optimal dilution should be determined by the end user. |
| Shipping | Shipped on dry ice. |
| Storage | Store at 4 °C: short-term (1-2 weeks) Store at -20 °C: long-term and future use |
| Buffer | PBS, 0.2% Gelatin |
For Research Use Only. Not For Clinical Use.Related Products
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