C. elegans ced-13 Functional Gene Editing Service

Service Overview Precision editing of CED-13 in a premier genetic model for cell death research

ced-13 encodes an apoptosis-related factor that contributes to DNA-damage-induced germline apoptosis and is partially redundant with the anti-apoptotic CED-9. Its emerging role in cell-death specificity makes it a valuable complement to the core CED-3 / CED-4 / CED-9 / EGL-1 axis.

Because ced-13 acts in a tissue-specific (germline) and signal-specific (DNA-damage) context, CRISPR-based alleles are ideal for clean dissection. CD BioSciences delivers knockouts, fluorescent tag knockins, and crosses into cep-1 or ced-9 mutant backgrounds.

About the Target Gene: ced-13 Key biological and practical facts to inform your edit design

Gene symbol	ced-13 (Apoptosis-related; DNA-damage-induced germline death)
Protein product	CED-13 — apoptosis-related factor in DNA-damage-induced germline apoptosis
Mammalian homolog	No clear mammalian ortholog.
Biological role	Apoptosis-related; implicated in DNA-damage-induced germline cell death; partially redundant with CED-9.
Loss-of-function phenotype	Reduced DNA-damage-induced germline apoptosis; subtle somatic apoptosis phenotypes due to redundancy with ced-9.
Why edit it	DNA damage response and germline apoptosis; cell death specificity; redundant anti-apoptotic regulation.
Recommended background	N2 (wild type); can be combined with ced-9 or cep-1 mutants for epistasis.
Representative allele	Standard knockout (ok / tm / gk series); reference alleles available on request.

Fig. 1 Conserved apoptosis pathway in C. elegans. The highlighted (amber) node marks the editing target.

ced-13 at a glance: an apoptosis-related factor contributing to DNA-damage-induced germline cell death; partially redundant with ced-9. Editing ced-13 alone is a powerful way to dissect CED-13 function in vivo, with conserved relevance to human No clear mammalian ortholog. biology.

Our ced-13 Editing Strategies Choose the allele that matches your experimental question

Gene Knockout

gRNA-guided Cas9 cutting with NHEJ repair to introduce frameshift indels, generating premature stop codons and null / strong-loss-of-function ced-13 alleles.

Knockin & Tagging

Homology-directed repair (HDR) inserts GFP / HA / FLAG or other tags at the ced-13 locus for endogenous expression and subcellular localization studies.

Point Mutation

Introduce specific amino-acid substitutions to dissect functional domains and separate catalytic from scaffolding roles of CED-13.

Conditional & Reporter Alleles

Build tissue-specific or inducible configurations and reporter lines for spatiotemporal analysis of CED-13 function.

Workflow of Our ced-13 Editing Service From sequence to validated strain in a streamlined pipeline

Strategy Design

Define target (knockout / knockin / point mutation), select gRNA(s) and HDR template, confirm against the latest genome build.

gRNA Synthesis

Design, validate, and synthesize high-specificity guide RNAs targeting the ced-13 locus.

Vector & Template Construction

Build Cas9 / gRNA delivery construct and, for knockin, the homology-arm repair template.

Microinjection

Perform gonadal microinjection of N2 animals with CRISPR components and co-CRISPR selection markers.

Screening & Verification

PCR, Sanger / sequencing, and phenotype confirmation to isolate edited animals and verify alleles.

Delivery & Report

Provide homozygous / balanced ced-13 strains plus a full genotype and methods report.

Key Features Why researchers choose CD BioSciences for C. elegans editing

High-efficiency, optimized CRISPR / Cas9 platform for C. elegans.
Whole-process technical traceability and transparent timelines.
Flexible editing: knockout, knockin, tagging, and point mutation.
Full genotype verification before strain delivery.
One-stop model development integrated with our C. elegans portfolio.
Competitive pricing and short turnaround.
Direct access to scientists and prompt inquiry response.
Reliable, convenient global shipping of frozen stocks.

Applications Where a custom ced-13 model adds value

DNA-damage-induced germline apoptosis.
Functional redundancy with ced-9 in cell-death regulation.
Cell-type and signal-specific apoptosis.
Cross-talk with the cep-1/p53 DNA-damage response axis.
Endogenous tagging for germline-specific localization.
Cancer / DNA-damage response modeling.

Deliverables What you receive with every project

Genetically verified ced-13 edited strain(s) (homozygous or balanced).
Detailed genotyping report (editing strategy, allele sequence, verification data).
Methods summary and rearing / storage recommendations.
Optional: crosses into reporter or co-CRISPR selection backgrounds.
Optional: phenotype confirmation and imaging support.

Frequently Asked Questions

What type of ced-13 allele will I get from a knockout project?

Standard knockout projects deliver a frameshift indel allele predicted to produce a premature stop and a loss-of-function phenotype. We confirm the edit by sequencing and, where relevant, by characteristic phenotype assays.

Can you add a GFP or epitope tag to endogenous ced-13?

Yes. Using HDR with a designed repair template, we can insert GFP, HA, or FLAG tags at the C- or N-terminus while preserving endogenous promoter and regulation.

How long does a typical ced-13 editing project take?

Timelines vary with editing type and validation needs, but our optimized pipeline and co-CRISPR selection typically shorten turnaround versus conventional approaches. Contact us with your design for a project-specific estimate.

Does ced-13 loss-of-function produce a visible somatic phenotype?

Somatic apoptosis is largely intact in ced-13 single mutants due to redundancy with ced-9. The clearest phenotypes appear in the germline under DNA-damage conditions (e.g. UV or ionizing radiation), so we recommend including such assays when characterizing new alleles.

Start Your ced-13 Editing Project

CD BioSciences is committed to convenient, efficient, and cost-effective C. elegans model customization. Tell us your target design — knockout, knockin, tagging, or point mutation — and our scientists will respond with a tailored plan and quote.

References
1. Kim H. M., Colaiácovo M. P. CRISPR-Cas9-guided genome engineering in C. elegans. Current Protocols in Molecular Biology, 2016, 115(1): 31.7.1–31.7.18.
2. Gartner A., Boag P. R., Blackwell T. K. Germline survival and apoptosis. WormBook, 2008, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.145.1.

For research use only.