C. elegans ced-11 Functional Gene Editing Service

Service Overview Precision editing of CED-11 in a premier genetic model for cell death research

ced-11 is an apoptosis-related gene in C. elegans linked to mitochondrial function. While it is less characterized than the canonical CED-3 / CED-4 / CED-9 / EGL-1 axis, it represents an emerging node in the cell-death network and an opportunity for first-of-its-kind genetic dissection.

Because ced-11 is not well covered by legacy alleles, CRISPR-based custom editing is the route of choice. CD BioSciences delivers clean knockouts, fluorescent tag knockins, and promoter / enhancer deletions to characterize the locus in apoptosis and mitochondrial contexts.

About the Target Gene: ced-11 Key biological and practical facts to inform your edit design

Gene symbol	ced-11 (Apoptosis-related mitochondrial factor)
Protein product	CED-11 — apoptosis-related mitochondrial factor
Mammalian homolog	No clear mammalian ortholog (under characterization).
Biological role	Implicated in apoptosis and mitochondrial function in C. elegans; less characterized than the core ced-3/ced-4/ced-9 axis.
Loss-of-function phenotype	Limited published characterization; emerging phenotype data suggest mild apoptosis-related defects.
Why edit it	An emerging apoptosis / mitochondrial factor; a first-of-its-kind model for a less-studied node in the cell-death network.
Recommended background	N2 (wild type); suitable for CRISPR-based knockout and tagging studies.
Representative allele	Standard knockout (ok / tm / gk series) on request.

Fig. 1 Conserved apoptosis pathway in C. elegans. The highlighted (amber) node marks the editing target.

ced-11 at a glance: an emerging apoptosis-related factor; clean CRISPR knockout enables first-of-its-kind loss-of-function analysis. Editing ced-11 alone is a powerful way to dissect CED-11 function in vivo, with conserved relevance to human No clear mammalian ortholog biology.

Our ced-11 Editing Strategies Choose the allele that matches your experimental question

Gene Knockout

gRNA-guided Cas9 cutting with NHEJ repair to introduce frameshift indels, generating premature stop codons and null / strong-loss-of-function ced-11 alleles.

Knockin & Tagging

Homology-directed repair (HDR) inserts GFP / HA / FLAG or other tags at the ced-11 locus for endogenous expression and subcellular localization studies.

Point Mutation

Introduce specific amino-acid substitutions to dissect functional domains and separate catalytic from scaffolding roles of CED-11.

Conditional & Reporter Alleles

Build tissue-specific or inducible configurations and reporter lines for spatiotemporal analysis of CED-11 function.

Workflow of Our ced-11 Editing Service From sequence to validated strain in a streamlined pipeline

Strategy Design

Define target (knockout / knockin / point mutation), select gRNA(s) and HDR template, confirm against the latest genome build.

gRNA Synthesis

Design, validate, and synthesize high-specificity guide RNAs targeting the ced-11 locus.

Vector & Template Construction

Build Cas9 / gRNA delivery construct and, for knockin, the homology-arm repair template.

Microinjection

Perform gonadal microinjection of N2 animals with CRISPR components and co-CRISPR selection markers.

Screening & Verification

PCR, Sanger / sequencing, and phenotype confirmation to isolate edited animals and verify alleles.

Delivery & Report

Provide homozygous / balanced ced-11 strains plus a full genotype and methods report.

Key Features Why researchers choose CD BioSciences for C. elegans editing

High-efficiency, optimized CRISPR / Cas9 platform for C. elegans.
Whole-process technical traceability and transparent timelines.
Flexible editing: knockout, knockin, tagging, and point mutation.
Full genotype verification before strain delivery.
One-stop model development integrated with our C. elegans portfolio.
Competitive pricing and short turnaround.
Direct access to scientists and prompt inquiry response.
Reliable, convenient global shipping of frozen stocks.

Applications Where a custom ced-11 model adds value

Characterization of an emerging apoptosis / mitochondrial factor.
First-of-its-kind ced-11 loss-of-function model.
Cross-talk between mitochondrial function and programmed cell death.
Endogenous tagging (GFP / mNeonGreen) for subcellular localization.
Promoter analysis to map regulatory elements.
Epistasis with the core ced-3/ced-4/ced-9/egl-1 axis.

Deliverables What you receive with every project

Genetically verified ced-11 edited strain(s) (homozygous or balanced).
Detailed genotyping report (editing strategy, allele sequence, verification data).
Methods summary and rearing / storage recommendations.
Optional: crosses into reporter or co-CRISPR selection backgrounds.
Optional: phenotype confirmation and imaging support.

Frequently Asked Questions

What type of ced-11 allele will I get from a knockout project?

Standard knockout projects deliver a frameshift indel allele predicted to produce a premature stop and a loss-of-function phenotype. We confirm the edit by sequencing and, where relevant, by characteristic phenotype assays.

Can you add a GFP or epitope tag to endogenous ced-11?

Yes. Using HDR with a designed repair template, we can insert GFP, HA, or FLAG tags at the C- or N-terminus while preserving endogenous promoter and regulation.

How long does a typical ced-11 editing project take?

Timelines vary with editing type and validation needs, but our optimized pipeline and co-CRISPR selection typically shorten turnaround versus conventional approaches. Contact us with your design for a project-specific estimate.

Is ced-11 functionally redundant with other ced genes?

Public data suggest ced-11 contributes to apoptosis-related phenotypes, but redundancy with the core axis has not been fully mapped. Knockout + epistasis with ced-3, ced-4, and ced-9 is the standard first-line approach.

Start Your ced-11 Editing Project

CD BioSciences is committed to convenient, efficient, and cost-effective C. elegans model customization. Tell us your target design — knockout, knockin, tagging, or point mutation — and our scientists will respond with a tailored plan and quote.

References
1. Kim H. M., Colaiácovo M. P. CRISPR-Cas9-guided genome engineering in C. elegans. Current Protocols in Molecular Biology, 2016, 115(1): 31.7.1–31.7.18.
2. Conradt B., Xue D. Programmed cell death. WormBook, 2005, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.32.1.

For research use only.