C. elegans ced-10 Functional Gene Editing Service

Service Overview Precision editing of CED-10 in a premier genetic model for cell death research

ced-10 encodes the C. elegans ortholog of mammalian Rac1 (RAC1/2/3). As the convergence point of both branches of the engulfment pathway, CED-10 integrates signals from the recognition branch (CED-1/-6/-7) and the signaling branch (CED-2/-5/-12) to drive actin polymerization around the apoptotic corpse.

Because Rac GTPases are central to cell migration, immune-cell function, and cancer metastasis in mammals, manipulating ced-10 directly informs these fields. CD BioSciences delivers knockout, GTPase-domain point mutants (constitutively active / dominant negative), and fluorescent knockins for Rac biology.

About the Target Gene: ced-10 Key biological and practical facts to inform your edit design

Gene symbol	ced-10 (Rac1 homolog GTPase (engulfment))
Protein product	CED-10 — Rac-family GTPase
Mammalian homolog	RAC1 / RAC2 / RAC3 (Rho-family GTPases)
Biological role	Rho-family GTPase that drives actin polymerization and cytoskeletal remodeling for corpse engulfment; the convergence point of both the recognition (CED-1/-6/-7) and signaling (CED-2/-5/-12) branches.
Loss-of-function phenotype	Persistent cell corpses; engulfment-defective; cell-migration defects.
Why edit it	Rac1 in cancer invasion, immunity, and NADPH oxidase regulation; actin dynamics in phagocytosis.
Recommended background	N2 (wild type); can be combined with ced-1, ced-2, ced-5, or ced-12 mutants.
Representative allele	Standard knockout (ok / tm / gk series); n1993 is a canonical reference allele.

Fig. 1 Recognition branch of the C. elegans corpse-clearance pathway. The highlighted (amber) node marks the editing target.

ced-10 at a glance: blocks engulfment of apoptotic corpses; persistent-cell-corpse phenotype easy to score. Editing ced-10 alone is a powerful way to dissect CED-10 function in vivo, with conserved relevance to human RAC1 biology.

Our ced-10 Editing Strategies Choose the allele that matches your experimental question

Gene Knockout

gRNA-guided Cas9 cutting with NHEJ repair to introduce frameshift indels, generating premature stop codons and null / strong-loss-of-function ced-10 alleles.

Knockin & Tagging

Homology-directed repair (HDR) inserts GFP / HA / FLAG or other tags at the ced-10 locus for endogenous expression and subcellular localization studies.

Point Mutation

Introduce specific amino-acid substitutions to dissect functional domains and separate catalytic from scaffolding roles of CED-10.

Conditional & Reporter Alleles

Build tissue-specific or inducible configurations and reporter lines for spatiotemporal analysis of CED-10 function.

Workflow of Our ced-10 Editing Service From sequence to validated strain in a streamlined pipeline

Strategy Design

Define target (knockout / knockin / point mutation), select gRNA(s) and HDR template, confirm against the latest genome build.

gRNA Synthesis

Design, validate, and synthesize high-specificity guide RNAs targeting the ced-10 locus.

Vector & Template Construction

Build Cas9 / gRNA delivery construct and, for knockin, the homology-arm repair template.

Microinjection

Perform gonadal microinjection of N2 animals with CRISPR components and co-CRISPR selection markers.

Screening & Verification

PCR, Sanger / sequencing, and phenotype confirmation to isolate edited animals and verify alleles.

Delivery & Report

Provide homozygous / balanced ced-10 strains plus a full genotype and methods report.

Key Features Why researchers choose CD BioSciences for C. elegans editing

High-efficiency, optimized CRISPR / Cas9 platform for C. elegans.
Whole-process technical traceability and transparent timelines.
Flexible editing: knockout, knockin, tagging, and point mutation.
Full genotype verification before strain delivery.
One-stop model development integrated with our C. elegans portfolio.
Competitive pricing and short turnaround.
Direct access to scientists and prompt inquiry response.
Reliable, convenient global shipping of frozen stocks.

Applications Where a custom ced-10 model adds value

Rac1 family signaling in cell migration and invasion.
Cancer invasion and metastasis modeling.
NADPH oxidase regulation and ROS biology.
Actin dynamics in phagocytosis and immune-cell function.
DOCK-Rac and ELMO-Rac signaling axes.
Convergence of the two engulfment branches.

Deliverables What you receive with every project

Genetically verified ced-10 edited strain(s) (homozygous or balanced).
Detailed genotyping report (editing strategy, allele sequence, verification data).
Methods summary and rearing / storage recommendations.
Optional: crosses into reporter or co-CRISPR selection backgrounds.
Optional: phenotype confirmation and imaging support.

Frequently Asked Questions

What type of ced-10 allele will I get from a knockout project?

Standard knockout projects deliver a frameshift indel allele predicted to produce a premature stop and a loss-of-function phenotype. We confirm the edit by sequencing and, where relevant, by characteristic phenotype assays.

Can you add a GFP or epitope tag to endogenous ced-10?

Yes. Using HDR with a designed repair template, we can insert GFP, HA, or FLAG tags at the C- or N-terminus while preserving endogenous promoter and regulation.

How long does a typical ced-10 editing project take?

Timelines vary with editing type and validation needs, but our optimized pipeline and co-CRISPR selection typically shorten turnaround versus conventional approaches. Contact us with your design for a project-specific estimate.

Can I make constitutively active or dominant-negative ced-10 alleles?

Yes. G12V (constitutively active) and T17N (dominant negative) are well-established in the C. elegans Rac literature. We can also build switch-I or switch-II point mutants to dissect GTP / GDP binding, Mg2+ coordination, and effector interactions.

Start Your ced-10 Editing Project

CD BioSciences is committed to convenient, efficient, and cost-effective C. elegans model customization. Tell us your target design — knockout, knockin, tagging, or point mutation — and our scientists will respond with a tailored plan and quote.

References
1. Kim H. M., Colaiácovo M. P. CRISPR-Cas9-guided genome engineering in C. elegans. Current Protocols in Molecular Biology, 2016, 115(1): 31.7.1–31.7.18.
2. Bosco E. E., Mulloy J. C., Zheng Y. Rac1 GTPase: a “Rac” of all trades. Cell Mol Life Sci, 2009, 66(23): 3701-3719.

For research use only.