C. elegans is a widely used model organism, and with the help of the gene editing tool CRISPR/Cas9, researchers are able to achieve precise gene knockout of the C. elegans genome to enable the study of specific functions of target genes. The success of knockout in C. elegans using CRISPR/Cas9 may vary depending on a number of factors, including the efficiency of sgRNA design, the expression level of Cas9, and the characteristics of the target gene. Optimizing these parameters can improve the success rate of knockout experiments.

As a new molecular biology technique for reverse genetics research, gene knockout mainly uses the principle of DNA homologous recombination to achieve gene knockout and is a common tool for biologists to understand gene function. CRISPR/Cas9-based gene knockout technology enables researchers to study the effects of deletion of specific genes, proteins of interest, amino acids, or regulatory elements to determine their function. The C. elegans gene knockout services we provide include frameshift, deletion, and conditional knockout services.

Fig.1 Generation of a deletion using the CRISPR/Cas9 protocol. (Au V, et al., 2019)

Our Services

As a powerful gene editing tool, CRISPR/Cas9 has revolutionized the study of genes in model organisms including C. elegans. For many years, CD BioSciences, relying on its extensive expertise in C. elegans genetics and gene editing technology, is confident in providing global customers with customized solutions for C. elegans gene knockout using CRISPR/Cas9 technology. We will work with you on the design of attractive projects to ensure you obtain the C. elegans strain that best suits your research needs. Our services include but are not limited to.

• C. elegans Full Gene Deletion Service

CD BioSciences has established a highly efficient CRISPR/Cas9 system-based technology platform for genome editing of C. elegans, which can quickly construct C. elegans strains. We can delete the entire coding sequence or precisely part of the coding sequence (a small defined region of DNA) of a gene of interest using CRISPR/Cas9, which will be useful for deleting protein domains, DNA regulatory regions, or whatever you are interested in other sequences.

• C. elegans Gene Conditional KO Service

C. elegans conditional knockout (KO) is the targeted inactivation of a gene in a specific tissue or at a specific developmental stage of your gene of interest and is a powerful tool for understanding the function of your gene of interest in different tissues. Our established CRISPR/Cas9-based technology platform makes C. elegans conditional KO easy.

• C. elegans Gene Partial Deletion/Frameshift Service

Frameshift mutations are genetic alterations that disrupt the reading frame of a gene, typically resulting in the production of a non-functional or truncated protein. Our experienced scientists strictly follow the use of CRISPR/Cas9 to induce specific frameshift mutations to ensure the correctness of the produced C. elegans strains.

Advantages of CD BioSciences

CD BioScience offers a broad and comprehensive portfolio of laboratory and production services, particularly custom service for C. elegans models. We have developed a proven CRISPR/Cas9-based gene editing technology platform that can be optimized to meet each of your specific requirements. We are committed to providing convenient and efficient shipping services with shorter turnaround times. If you are interested in our services or have any additional questions, please feel free to contact us.

Reference

1. Au V, Li-Leger E, Raymant G, et al. CRISPR/Cas9 methodology for the generation of knockout deletions in Caenorhabditis elegans[J]. G3: Genes, Genomes, Genetics, 2019, 9(1): 135-144.

Related Services

• C. elegans Gene Knockin by CRISPR/Cas9