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C. elegans has received much attention since its introduction in the early 1970s. The nematode has been instrumented as a platform for biological research and the implementation of a vast array of technologies. Studies have found that about 40% of genes known to be associated with human diseases have orthologs in the C. elegans genome. Since the antioxidant system between humans and C. elegans is similar, including similar antioxidant enzymes (Superoxide Dismutase, SOD; Catalase, CAT) and related genes (Glutathione S-transferase, GST and Peroxidase gene, PRDX), C. elegans has emerged as a suitable animal model to dissect the gene regulatory network involved in the expression of oxidative stress response genes.
High doses of reactive oxygen species (ROS) can cause oxidative stress that has been proposed to be one of the main causes of aging and implicated in the pathogenesis of many diseases. The primary source of ROS in most cells is from the mitochondrion due to its inefficiencies in oxidative phosphorylation. When ROS concentrations exceed the antioxidant capacity of the cell, severe DNA, lipid, and protein damage can be observed and organisms suffer from an oxidative stress condition. Environmental perturbations induced ROS was observed to lead to reduced levels of dopamine, an important neurotransmitter released by nerve cells. Previous research has established an approach to induce ROS using paraquat, a herbicide, and observed its effect on neurite outgrowth in C. elegans. At present, many chemical perturbations causing ROS have been used on C. elegans to establish a model for Parkinson's disease.
Oxidative stress service
ROS-inducing reagents like paraquat, hydrogen peroxide and sodium arsenite were used to establish an oxidative stress model to explore the genes and oxidative stress signal transduction pathways underlying oxidative stress-associated human disease, rendering potential targets for drug discovery.
| ROS-inducing Reagents | Process |
|---|---|
| Paraquat (PQ) | Paraquat are dissolved in M9 buffer, and solutions are aliquoted in the wells of a 24 well microtiter plate. Age-synchronized young adult (24 hpost-larval stage L4) C. elegans are transferred into the wells (5-8 animals per well) and survival is measured every 30 min until most worms are dead. |
| Hydrogen peroxide (H2O2) | Synchronized C. elegans aged 5 days are sampled in replicate groups of 50 adult animals and washed in magnesium-free M9 medium, after which they are incubated in M9 with the indicated concentration of H2O2 at 20 °C in for 4 h. The animals were seeded on fresh nematode growth medium plates and immediately singled. |
| Sodium arsenite | Synchronized L1 stage animals were collected by low speed centrifugation for five minutes and transferred to sodium arsenite containing (0.03% and 0.003% w/v) CeHM media and incubated at 22°C for 6 hours. |
CD BioSciences offers a broad and integrated portfolio of laboratory and manufacturing C. elegans oxidative stress services, ranging from C. elegans oxidative stress model system to kinds of antioxidant function detection for exploring the specific physiological consequences of oxidative stress conditions in higher eukaryotes, and providing potential targets for drug discovery. If you are interested in our services or have any questions about our services, please don't hesitate to contact us for more details.
References
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